FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000278912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677456
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC23620834.86691386599277No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTC23126834.137715216929216No Hit
ATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTAC229603.389149996457334No Hit
ACGAGATGATCAGCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT168012.480013462128906Illumina Paired End PCR Primer 2 (97% over 39bp)
AATATGCATATTAAAGCAAGATTTACCTCTATTGTTGGATCATATTCGTC157592.326202734937767No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC157522.3251694574998227No Hit
GTAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCG54510.80462790203349No Hit
AAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGT20440.3017170118797383No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC18780.27721357549420184No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA14820.2187595947190666No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC14590.21536454028010676No Hit
ATCAACGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC12360.18244727332845234No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTCGGATCATATTCGTC12020.17742849720129425No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGCC11770.17373822063720742No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC11270.1663576675090338No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGAC10750.15868189225573323No Hit
ATACGAGATGATCAGCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGAT10620.15676294844240807Illumina Paired End PCR Primer 2 (97% over 37bp)
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACACGATTTAC10280.15174417231525No Hit
ATCAAAGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC10010.14775867362603623No Hit
ATCAAAGAATGGTCCTGCACCCGTAATATGCATATTAAAACAAGATTTAC9960.14702061831321886No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGATTTAC9730.14362556387425898No Hit
ATCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC8900.13137384568149077No Hit
ATCATAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC8860.13078340143123687No Hit
AATATGCGTATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTC8760.12930729080560213No Hit
AATATGCATATTAAAACAAGATTTACCCCTATTGTTGGATCATATTCGTC8140.12015540492666683No Hit
TCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC8090.11941734961384946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7510.11085590798516805No Hit
ANTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTAC7490.11056068586004109No Hit
ATCCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7320.10805129779646205No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGACCATATTCGTC7160.10568952079544651No Hit
ATCAAAGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7040.10391818804468482No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCGTATTCGTC6860.1012611889185423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)