FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000278913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178000
Sequences flagged as poor quality0
Sequence length141-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC12848872.18426966292135No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC81304.567415730337078No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC29101.6348314606741572No Hit
ATCTAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC26241.4741573033707867No Hit
TTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC18131.0185393258426967No Hit
ACCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC11600.651685393258427No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC9820.551685393258427No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA8520.4786516853932585No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC6090.34213483146067414No Hit
AACAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5880.3303370786516854No Hit
ATCAAAGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5430.3050561797752809No Hit
ATCATAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4970.2792134831460674No Hit
AGCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4810.2702247191011236No Hit
ATCCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4700.2640449438202247No Hit
ATCAACGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4520.2539325842696629No Hit
ATCAAAGAATGGTCCTGCACCAGTAATACGCATATTAAAACAAGATTTAC4400.24719101123595505No Hit
ATCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC4310.24213483146067416No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGATTTAC3840.21573033707865166No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCACATTAAAACAAGATTTAC3800.21348314606741575No Hit
ATCAAAGAATGGTGCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3700.20786516853932585No Hit
ATCAAAGAATGGCCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3680.20674157303370785No Hit
ATCGAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3640.2044943820224719No Hit
ATCAAATAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3570.200561797752809No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATACTAAAACAAGATTTAC3560.2No Hit
ATCAAAGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3470.1949438202247191No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGGTTTAC3430.19269662921348316No Hit
ATCAAAGAATGGTCCTGCACCCGTAATATGCATATTAAAACAAGATTTAC3050.17134831460674158No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGTCTATCAA2890.16235955056179774No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACGAGATTTAC2800.15730337078651685No Hit
ATCAAAGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGATTTAC2730.15337078651685393No Hit
ATCAAAGAATGGTCCTGCATCAGTAATATGCATATTAAAACAAGATTTAC2560.14382022471910114No Hit
ATCAAAGAATGGTCCTGTACCAGTAATATGCATATTAAAACAAGATTTAC2530.14213483146067415No Hit
TCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC2430.13651685393258425No Hit
ATCAAAGAATGGTCCTGCACCGGTAATATGCATATTAAAACAAGATTTAC2310.1297752808988764No Hit
ATCAAAGAATGGTCCTGCACCAGTAACATGCATATTAAAACAAGATTTAC2230.1252808988764045No Hit
ATCAAAGAATGGTCCTGAACCAGTAATATGCATATTAAAACAAGATTTAC2130.11966292134831462No Hit
ATCAAAGAATGGTCCTGCACCAGTAGTATGCATATTAAAACAAGATTTAC2110.11853932584269664No Hit
GTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2060.11573033707865168No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACACGATTTAC2040.1146067415730337No Hit
ATCAAAGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1980.11123595505617978No Hit
ATCAAAGAATGGTCCTGCCCCAGTAATATGCATATTAAAACAAGATTTAC1950.10955056179775281No Hit
ATCAAAGAATGGACCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1890.10617977528089888No Hit
ATCAAAGAATGGTCCTGCACCAGTAATGTGCATATTAAAACAAGATTTAC1830.10280898876404494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)