FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000278914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273581
Sequences flagged as poor quality0
Sequence length141-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC19921672.81792229723555No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC123204.503236701379116No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC43161.577594935320801No Hit
ATCTAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC39381.439427445619396No Hit
TTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC28461.040276919815338No Hit
ACCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC16570.60567071543711No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC14610.534028313369715No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA12200.44593740062358134No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC9360.342129022117764No Hit
AACAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC8360.30557677616501144No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGATTTAC8050.29424557991965816No Hit
ATCAAAGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC8020.29314901254107556No Hit
ATCATAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7880.28803169810769025No Hit
ATCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC7750.28327990613383236No Hit
AGCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7690.28108677137666727No Hit
ATCAACGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7380.26975557513131393No Hit
ATCCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC6600.24124482328816693No Hit
ATCAAAGAATGGCCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC6020.22004452063557042No Hit
ATCAAAGAATGGTCCTGCACCAGTAATACGCATATTAAAACAAGATTTAC5950.2174858634188778No Hit
ATCGAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5530.20213392011872172No Hit
ATCAAAGAATGGTCCTGCATCAGTAATATGCATATTAAAACAAGATTTAC5130.18751302173762066No Hit
ATCAAAGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5010.18312675222329036No Hit
ATCAAAGAATGGTCCTGCACCCGTAATATGCATATTAAAACAAGATTTAC5010.18312675222329036No Hit
ATCAAATAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5000.18276122976376283No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATACTAAAACAAGATTTAC4970.18166466238518025No Hit
ATCAAAGAATGGTGCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4960.18129913992565272No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACACGATTTAC4770.17435421319462974No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCACATTAAAACAAGATTTAC4700.17179555597793705No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGGTTTAC4690.17143003351840952No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGTCTATCAA4100.14986420840628553No Hit
TCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC4010.14657450627053778No Hit
ATCAAAGAATGGTCCTGTACCAGTAATATGCATATTAAAACAAGATTTAC3890.14218823675620748No Hit
ATCAAAGAATGGTCCTGCACCAGCAATATGCATATTAAAACAAGATTTAC3880.14182271429667997No Hit
ATCAAAGAATGGTCCTGCACCGGTAATATGCATATTAAAACAAGATTTAC3760.13743644478234965No Hit
ATCAAAGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGATTTAC3450.12610524853699637No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAGGATTTAC3410.12464315869888626No Hit
ATCAAAGAATGGTCCTGAACCAGTAATATGCATATTAAAACAAGATTTAC3230.11806375442739078No Hit
GTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3120.114043007372588No Hit
ATCAAAGATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC2990.10929121539873018No Hit
ATCAAAGAATGGTCCTGCCCCAGTAATATGCATATTAAAACAAGATTTAC2980.10892569293920265No Hit
ATCAAAGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2960.10819464802014758No Hit
ATGAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2930.10709808064156502No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTCAC2910.10636703572250997No Hit
ATCAAAGAATGGACCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2870.10490494588439986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)