FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000278917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333354
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC46165434.6235133355433No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTC44507633.38018260716959No Hit
ATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTAC551294.134610913530841No Hit
ACGAGATGATCAGCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT407243.054252659083784Illumina Paired End PCR Primer 2 (97% over 39bp)
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC293442.200765888128734No Hit
AATATGCATATTAAAGCAAGATTTACCTCTATTGTTGGATCATATTCGTC283122.123367087810139No Hit
GTAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCG96940.7270387308996711No Hit
AAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGT43930.32946989321665515No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC36380.27284577089055123No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC25850.19387199498407776No Hit
ATCAACGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC24500.18374715191914526No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC23750.17812223910529387No Hit
ATACGAGATGATCAGCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGAT23660.17744724956763172Illumina Paired End PCR Primer 2 (97% over 37bp)
ANTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTAC23260.17444729606691095No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA23080.17309731699158665No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGAC22980.17234732861640645No Hit
ATCAAAGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC19870.1490226901483027No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGCC19420.14564774245999187No Hit
ATCAAAGAATGGTCCTGCACCCGTAATATGCATATTAAAACAAGATTTAC18970.14227279477168103No Hit
ATCATAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC17010.12757302261814943No Hit
ATCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC16580.12434807260487463No Hit
TCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC15310.11482322024008627No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15280.11459822372753223No Hit
ATCCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC14920.11189826557688357No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACACGATTTAC14680.11009829347645111No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGACCATATTCGTC14540.10904830975119886No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGATTTAC14480.10859831672609074No Hit
ATCAAAGAATGGTCCTGCACCAGTAATACGCATATTAAAACAAGATTTAC14070.105523364387852No Hit
AATATGCGTATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTC13920.10439838182508172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)