FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000278920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91887
Sequences flagged as poor quality0
Sequence length47-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC6565071.44645053163124No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC44534.846169751977973No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC14921.6237334987539043No Hit
ATCTAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC13911.5138158825513945No Hit
TTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC10271.117677146930469No Hit
ACCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC6370.6932427873366199No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC5170.5626475997692819No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA4320.4701426752424173No Hit
AACAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3230.35151871320208516No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3200.34825383351290173No Hit
ATCAAAGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2940.31995820953997844No Hit
ATCAACGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2850.3101635704724281No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGATTTAC2780.30254551786433337No Hit
ATCATAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2590.2818679464995048No Hit
AGCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2400.2611903751346763No Hit
ATCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC2350.2557489089860372No Hit
ATCAAAGAATGGTCCTGCACCAGTAATACGCATATTAAAACAAGATTTAC2340.2546606157563094No Hit
ATCCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2340.2546606157563094No Hit
ATCGAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2140.232894751161753No Hit
ATCAAAGAATGGTCCTGCACCCGTAATATGCATATTAAAACAAGATTTAC1880.20459912718882975No Hit
ATCAAAGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1800.19589278135100724No Hit
ATCAAAGAATGGTCCTGCACCGGTAATATGCATATTAAAACAAGATTTAC1740.18936302197264032No Hit
ATCAAAGAATGGCCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1720.18718643551318467No Hit
ATCAAAGAATGGTGCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1640.17848008967536214No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACGAGATTTAC1640.17848008967536214No Hit
ATCAAATAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1550.1686854506078118No Hit
ATCAAAGAATGGTCCTGCATCAGTAATATGCATATTAAAACAAGATTTAC1500.16324398445917268No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAGGATTTAC1390.15127275893216668No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGGTTTAC1380.15018446570243887No Hit
TCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC1350.14691958601325542No Hit
ATCAAAGAATGGTCCTGTACCAGTAATATGCATATTAAAACAAGATTTAC1340.14583129278352758No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGTCTATCAA1340.14583129278352758No Hit
GTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1320.14365470632407196No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCACATTAAAACAAGATTTAC1300.14147811986461634No Hit
ATCAAAGAATGGTCCTGCACCAGCAATATGCATATTAAAACAAGATTTAC1250.13603665371597723No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTCAC1220.13277177402679377No Hit
ATCAAAGAATGGTCCTGCCCCAGTAATATGCATATTAAAACAAGATTTAC1150.12515372141869904No Hit
ATCAAAGAATGGTCCTGAACCAGTAATATGCATATTAAAACAAGATTTAC1100.11971225527005996No Hit
ATCAAAGAATGGTCCTGCACCAGTAGTATGCATATTAAAACAAGATTTAC1080.11753566881060433No Hit
ATCAAAGAATGGACCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1070.1164473755808765No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC1020.11100590943223741No Hit
ATGAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC970.10556444328359833No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATACTAAAACAAGATTTAC960.10447615005387051No Hit
ATCAAAGAATGGTCCTGCGCCAGTAATATGCATATTAAAACAAGATTTAC930.10121127036468708No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTGC930.10121127036468708No Hit
ATCAAAGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGATTTAC920.10012297713495924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)