FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000278935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43412
Sequences flagged as poor quality0
Sequence length21-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGCTCTGATAGGAAAAT2025146.648392149636045No Hit
TCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT1942844.75260296692159No Hit
TCCACAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATC930.2142264811572837No Hit
TCCTCAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT870.20040541785681382No Hit
TCTACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT820.1888878651064222No Hit
TCCACAAAATGGATCTAGACAACTGTTCAAACTGATGGGACCCACTCCAT810.18658435455634387No Hit
TCCACAAACTGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT800.18428084400626554No Hit
TCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATC750.17276329125587395No Hit
TTCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT700.16124573850548235No Hit
TCCACAAAATGGATCCAGCCAACTGTTCAAACTGATGGGACCCACTCCAT700.16124573850548235No Hit
TGCTTGCTCTGATAGGAAATG600.13821063300469916No Hit
TCCACTAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT530.12208605915415091No Hit
CCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATC500.11517552750391596No Hit
TCCACAAAATGGATCCATACAACTGTTCAAACTGATGGGACCCACTCCAT470.108264995853681No Hit
TCCACAAAAGGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT440.10135446420344604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)