FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000278936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559330
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT23403841.84256163624337No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCA22548140.31269554645737No Hit
TCCACAAAATGGATCCAGACACCATTTTCCTATCAGAGCAAGCA107731.9260543864981317No Hit
ACTGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGA84891.5177086871793037No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAAACCA34250.6123397636457905No Hit
CAACTGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGACC15280.27318398798562565No Hit
TCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACCCCAT14230.25441152807823647No Hit
TCCACAAAATGGATCCAGACAACTGCTCAAACTGATGGGACCCACTCCAT11720.2095364096329537No Hit
GGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACC11250.2011334990077414No Hit
GTTCAAACTGATGGGACCCACCCCATCGAGATTTCACTGTAGCTAGACCA10640.19022759372821055No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGGCCA10510.1879033844063433No Hit
TCCTCAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT10500.18772459907389197No Hit
TCCACAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATC10120.1809307564407416No Hit
TCTACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT9990.17860654711887436No Hit
TCCACAAACTGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT9580.17127634848837003No Hit
TCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATC9180.16412493519031698No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCCAGACCA9040.16162194053599843No Hit
TCCACAAAATGGATCTAGACAACTGTTCAAACTGATGGGACCCACTCCAT8750.15643716589491No Hit
TTCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT8740.15625838056245867No Hit
TCCACAAAATGGATCCAGACAACTGTTCGAACTGATGGGACCCACTCCAT8420.15053724992401624No Hit
TCCACAAAATGGATCCAGACAGCTGTTCAAACTGATGGGACCCACTCCAT8170.1460676166127331No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGATTTCACCGTAGCTAGACCA8120.14517368995047647No Hit
TCCACAAAATGGATCCAGCCAACTGTTCAAACTGATGGGACCCACTCCAT7590.1356980673305562No Hit
GTTCAGACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCA7200.1287254393649545No Hit
GTTCGAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCA7100.12693758604044122No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGACTTCACTGTAGCTAGACCA6960.12443459138612269No Hit
GCTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCA6840.12228916739670677No Hit
TCCACAAAATGGATCCAGACAACTGTTCAGACTGATGGGACCCACTCCAT6680.11942860207748557No Hit
GTTCAAACTGATGGGACCCACTCCGTCGAGATTTCACTGTAGCTAGACCA6570.11746196342052098No Hit
TCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCGT6360.11370747143904314No Hit
TCCACTAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT6350.11352868610659181No Hit
CCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATC6350.11352868610659181No Hit
TCCACAAAATGGATCCATACAACTGTTCAAACTGATGGGACCCACTCCAT6330.11317111544168917No Hit
GTTCAAACTGATGGAACCCACTCCATCGAGATTTCACTGTAGCTAGACCA6160.11013176479001663No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAACTAGACCA6140.10977419412511397No Hit
GTTCAAACTGATGGGACCCACTCCATCGAGATCTCACTGTAGCTAGACCA5840.1044106341515742No Hit
GTTCAAACGGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCA5810.10387427815422023No Hit
TCCACAAAATGGATTCAGACAACTGTTCAAACTGATGGGACCCACTCCAT5660.10119249816745034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)