FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000278952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399140
Sequences flagged as poor quality0
Sequence length39-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTC13615434.11184045698251No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNAATCACTGAGCAGGAG289597.255349000350754No Hit
NNNNNNNNNNNNNNNNACGNNNNCNNNNNNNTGAAATCACTGAGCAGGAG185464.64648995339981No Hit
NNNNNNNNNNNNNNNNACNNNNNCNNNNNNNNGAAATCACTGAGCAGGAG90282.2618630054617426No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNAATCACTGCGCAGGAG72751.8226687377862403No Hit
NNANNNNNNNNNNNNNACGNNNTCNNNNCNCTGAAATCACTGAGCAGGAG47771.1968231698151024No Hit
NNNNNNNNNNNNNNNNNCNNNNNNNNNNCNCNGAAATCACTGAGCAGGAG47041.1785338477727112No Hit
NNNNNNNNNNNNNNNNACNNNNNCNNCNNNNTGAAATCACTGAGCAGGAG45551.1412035877135842No Hit
NNNNNNNNNNNNNNNNNCNNNNNNNNNNNNCNGNAATCACTGAGCAGGAG45411.1376960464999748No Hit
NNANNNNNNNNNNNNNACGNNNNCCNCNNNNTGAAATCACTGAGCAGGAG45261.1339379666282507No Hit
NNNNNNNNNNNNNNNNACGNNNNCNNNNNNNTGAAATCACTGCGCAGGAG44891.1246680362779977No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNANTCACTGAGCAGGAG44421.1128927193465952No Hit
NNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNGAAATCACTGAGCAGGAG44021.1028711730219973No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACTGAGCAGGAG41481.0392343538608007No Hit
NNNNNNNNNNNNNNNNACNNNNNCNNNNCNCTGAAATCACTGAGCAGGAG40741.0206944931602946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCACTGAGCAGGAG38730.9703362228791902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTCACTGAGCAGGAG37580.9415242771959714No Hit
NCNNNNNNNNNNNNNCACGNNNTCCNNNNTNTGAAATCACTGAGCAGGAG36360.9109585609059478No Hit
NNNNNNNNNNNNNNNNACGNNNGCNNNNNNCTGAAATCACTGAGCAGGAG33920.8498271283259007No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNATCACTGAGCAGGAG30170.7558751315327955No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCACTTTCTACACGAGATCCTCTC27920.6995039334569324No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNCNNNCNGNAATCACTGAGCAGGAGA26610.6666833692438743No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTCCACGAGATCCTCTC25320.6343638823470461No Hit
NNNNNNNNNNNNNNNNACNNNNNCNNNNNNNNGAAATCACTGCGCAGGAG22520.5642130580748609No Hit
NNANNNNNNNNNNNNNACGNNNTCNNNNCNCTGAAATCACTGCGCAGGAG12240.30665931753269526No Hit
NNNNNNNNNNNNNNNNACNNNNNCNNCNNNNTGAAATCACTGCGCAGGAG11800.29563561657563764No Hit
NNNNNNNNNNNNNNNNNCNNNNNNNNNNCNCNGAAATCACTGCGCAGGAG11710.2933807686526031No Hit
NNANNNNNNNNNNNNNACGNNNNCCNCNNNNTGAAATCACTGCGCAGGAG11700.2931302299944882No Hit
NNNNNNNNNNNNNNNNACGNNNTCNNNNNNCTGAAATCACTGAGCAGGAG11560.2896226887808789No Hit
NNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNGAAATCACTGCGCAGGAG11450.28686676354161444No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNANTCACTGCGCAGGAG11300.2831086836698902No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGCTCCTCTC10760.26957959613168314No Hit
NNNNNNNNNNNNNNNNNCNNNNNNNNNNNNCNGNAATCACTGCGCAGGAG10730.2688279801573383No Hit
GGGAAAATGACAAAGAACAGCTCAAATCAATTTCTACACGAGATCCTCTC10270.2573032018840507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACTGCGCAGGAG10130.25379566067044146No Hit
NNNNNNNNNNNNNNNNACNNNNNCNNNNCNCTGAAATCACTGCGCAGGAG10030.251290274089292No Hit
GGGAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCT9580.24001603447411937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTCACTGCGCAGGAG9560.23951495715788945No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCACTGCGCAGGAG9280.23249987473067096No Hit
NCNNNNNNNNNNNNNCACGNNNTCCNNNNTNTGAAATCACTGCGCAGGAG9050.22673748559402718No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCCC8970.22473317632910758No Hit
NNNNNNNNNNNNNNNNACGNNNGCNNNNNNCTGAAATCACTGCGCAGGAG8400.2104524728165556No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNATCACTGCGCAGGAG7560.18940722553490003No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNAAATCACTGAGCAGGA7190.180137295184647No Hit
GGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCT7180.17988675652653205No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACCCGAGATCCTCTC6790.1701157488600491No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGATCACTGAGCAGGAG6750.16911359422758931No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNCNNNCNGNAATCACTGCGCAGGAGA6510.16310066643283058No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNATCACTGAGCAGGAG6190.15508342937315228No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCCACACGAGATCCTCTC5750.1440597284160946No Hit
GGGAAAATGACAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCT5530.13854787793756576No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGTGATCCTCTC5310.13303602745903695No Hit
NANNNNNNNNNNNNNCACGNNNTCCNNNNTNTGAAATCACTGAGCAGGAG5310.13303602745903695No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCATCTC4970.12451771308312873No Hit
GGGAAAATGACAAAGAACAGTTCAAAGCAATTTCTACACGAGATCCTCTC4870.12201232650197927No Hit
NNNNNNNNNNNNNNNNACNNNNNCNNNNCNCAGAAATCACTGAGCAGGAG4640.11624993736533548No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTCCTACACGAGATCCTCTC4630.11599939870722052No Hit
GGGAAAATGACAAAGAACAGCCCAAAGCAATTTCTACACGAGATCCTCTC4620.11574886004910556No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACGCGAGATCCTCTC4540.11374455078418601No Hit
NNNNNNNNNNNNNNNNCACNNNNTNNNNNNNCTGAAATCACTGAGCAGGA4520.1132434734679561No Hit
NCNNNNNNNNNNNNNCACGNNNTCCNNNNANTGAAATCACTGAGCAGGAG4450.11148970286115148No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATCTCTACACGAGATCCTCTC4410.1104875482286917No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCAC4390.10998647091246179No Hit
NNANNNNNNNNNNNNNACGNNNGCNNNNCNCTGAAATCACTGAGCAGGAG4360.10923485493811697No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNATCACTGAGCAGGAGAA4330.10848323896377211No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGATATCCTCTC4190.10497569775016284No Hit
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACCAGATCCTCTC4170.10447462043393296No Hit
GGGAAAATGACAACGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTC4130.10347246580147315No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNAATCACTGAGAAGGAG4120.10322192714335822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)