FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000278963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154837
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT6544042.26379999612496No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG5818537.578227426261165No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAACAAATNAATGATGCACATCATG20741.3394731233490702No Hit
CTGAGCAAGAGGCTTTGGAGTCAAAGCTCACTCTGGATTCCA13290.858321977305166No Hit
AGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCAT11190.7226954797625891No Hit
CTGAGCAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC3730.24089849325419638No Hit
AGCAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG3170.20473142724284246No Hit
GCAAGAGGCGTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2970.19181461795307325No Hit
GCAAGAGGCTGTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2710.17502276587637322No Hit
GAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCA2570.16598099937353475No Hit
CTGGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC2540.16404347798006938No Hit
CTGAGCAAGAGGCTTGGAGTATTTCATGAAACAAATGAATGATGCACATC2500.16146011612211553No Hit
CTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAGTGATGCACAT2250.14531410450990395No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCGCATCATG2160.1395015403295078No Hit
GTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT2140.13820985940053088No Hit
CTGAGCAAGAGGCTTTGGAGTATTTCATGAAGCAAATGAATGATGCACAT2130.13756401893604242No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAGCAAATGAATGATGCACATCATG2010.1298139333621809No Hit
AGCAAGAGGCTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2010.1298139333621809No Hit
CTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCGCAT1960.12658473103973855No Hit
GCAAGATGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1950.1259388905752501No Hit
CTGAGAAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT1880.12141800732383087No Hit
GCAAGAGGTTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1870.1207721668593424No Hit
CTAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1870.1207721668593424No Hit
CTGAGCAAGAGGTTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT1850.11948048593036548No Hit
CAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATGG1800.11625128360792318No Hit
ACAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1780.11495960267894625No Hit
CTGAGCAAGAGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1770.11431376221445778No Hit
CTGAGCAAGAGGCTTTGGAGTATTTCATGAGACAAATGAATGATGCACAT1730.11173040035650393No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1690.10914703849855009No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAGTGATGCACATCATG1600.10333447431815394No Hit
GCAATAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1570.10139695292468855No Hit
AGCAAGAGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1560.10075111246020008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)