FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000278971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156111
Sequences flagged as poor quality0
Sequence length48-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT6715843.01939004938794No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG6097039.05554381177495No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAACAAATNAATGATGCACATCATG21311.3650543523518521No Hit
AGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCAT9230.5912459724170622No Hit
CTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGGATGATGCACAT6990.4477583258066376No Hit
GCAAGAGGCTTTGGAGTATTTCATGAAACAAATGGATGATGCACATCATG6600.4227761016200012No Hit
CTGAGCAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC3780.24213540365509156No Hit
AGCAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG3270.2094663412571824No Hit
CTGAGCAAGAGGCTTGGAGTATTTCATGAAACAAATGAATGATGCACATC2780.178078418561152No Hit
CTGGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC2520.1614236024367277No Hit
GCAAGAGGCGTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2420.15501790392733375No Hit
GCAAGAGGCTGTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2370.15181505467263678No Hit
AGCAAGAGGCTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2360.15117448482169737No Hit
GTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT2340.14989334511981858No Hit
CTGAGCAAGAGGCTTTGGGGTATTTCATGAAACAAATGAATGATGCACAT2300.147331065716061No Hit
ACAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2240.14348764661042465No Hit
GCAAGAGGTTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG2010.12875454003881853No Hit
CTGAGCAAGAGGTTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT2010.12875454003881853No Hit
GCAAGAGGCTTTGGGGTATTTCATGAAACAAATGAATGATGCACATCATG1970.12619226063506095No Hit
CTAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1940.12427055108224275No Hit
GAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCA1900.12170827167848518No Hit
CTGAGAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1870.119786562125667No Hit
CTGAGCAAGAGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1870.119786562125667No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1770.11338086361627304No Hit
AGAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1760.11274029376533365No Hit
GCAAGATGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1710.10953744451063666No Hit
CAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATGG1650.1056940254050003No Hit
CGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1640.10505345555406088No Hit
AGCAAGAGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1620.1037723158521821No Hit
GCAATAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATG1610.10313174600124271No Hit
CTGAGCAAGGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1600.1024911761503033No Hit
TTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT1580.10121003644842452No Hit
CTGAGAAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACAT1570.10056946659748511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)