FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000278976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680054
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA49025572.09059868775127No Hit
TGCTATGCAAATACAATAAACTGGAGTTGGTTCTAATGGGTCC7728611.364685745543737No Hit
TGCTATGCAAATACAATAAACTGGGTGACTGGAGTTGGTTCTAATGGGTCC66330.9753637211162643No Hit
TGCTATGCAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAG21080.30997538430771676No Hit
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGGCCTCCGGTCA19170.2818893793728145No Hit
TGCTATGCAAATACAATAAACTGGGGGAGTTGGTTCTAATGGGTCC16640.24468645137003825No Hit
GCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAG16280.23939275410482108No Hit
TGCTATGCAAATACAATAACTGGAAAAAACTGTTTGGGACCTCCGGTCAG13960.20527781617342153No Hit
TGCTATGCAAATACAATAAACTGGAGTTGGTTCTAATGGGTCCAGATCGG13710.20160163751702073No Hit
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGCCA13270.19513156308175528No Hit
TGCTATGCAAATCCAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA12420.1826325556499925No Hit
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCGCCGGTCA12290.1807209427486641No Hit
TGCTATGCAAATACCATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA12220.17969161272487186No Hit
TGCTATGCAAATACATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAG11630.17101583109576593No Hit
TGCTCTGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA11480.16881012390192543No Hit
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCG10900.16028138941907555No Hit
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCCCCGGTCA10850.15954615368779537No Hit
TGCTATGCAAATACAATAAACTGGGAAAAACTGTTTGGGACCTCCGGTCA10680.15704635220144283No Hit
TGCGATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA9350.1374890817493905No Hit
CTCCGATCTTGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGAC9340.13734203460313446No Hit
TGCTATGCACATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA9250.13601861028683015No Hit
CTTCGATCTTGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGAC9240.13587156314057414No Hit
TGCTATGCAAAGACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA9220.13557746884806207No Hit
TGTTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA8690.12778397009649234No Hit
TGCTATCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAG8540.12557826290265187No Hit
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTCGGGACCTCCGGTCA8530.12543121575639582No Hit
TGCTATGCAAATACACTAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA8390.12337255570881137No Hit
TGCTATGCAAATACAATAAATTGGAAAAAACTGTTTGGGACCTCCGGTCA8350.12278436712378724No Hit
TGCTATGCAAATACAATAAACTGGAGAAAACTGTTTGGGACCTCCGGTCA8170.12013751849117864No Hit
TGCTATGCAAATCAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAG8040.11822590558985022No Hit
CGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCA7960.11704952841980196No Hit
TGCTATGCAAATACAATAAACTGGAAAAAGCTGTTTGGGACCTCCGGTCA7590.11160878400832874No Hit
TGCTATGCAAATACAATAAACTTGAAAAAACTGTTTGGGACCTCCGGTCA7590.11160878400832874No Hit
TGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCAGTCA7400.10881488822946414No Hit
TGCTATGCAAATACAATAAACTGGTGACTGGAGTTGGTTCTAATGGGTCC7120.10469756813429522No Hit
TGCTATGCAAATACAATTAACTGGAAAAAACTGTTTGGGACCTCCGGTCA6910.10160957806291852No Hit
TCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAG6870.10102138947789441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)