FastQCFastQC Report
Fri 24 Mar 2023
EGAF00000286243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000286243
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences104478095
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT5199690.4976823132159904Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA4803180.4597308172588714Illumina Paired End PCR Primer 2 (100% over 45bp)
GTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC1713740.16402864160185923Illumina Paired End PCR Primer 2 (100% over 58bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC1371790.13129929292834064Illumina Paired End PCR Primer 2 (100% over 55bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)