FastQCFastQC Report
Thu 16 May 2024
EGAF00000286244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000286244
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences74114716
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG3782830.5104020097709071Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2674850.36090673274657087Illumina Paired End PCR Primer 2 (97% over 36bp)
GTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG1113230.15020363837054979Illumina Paired End PCR Primer 2 (100% over 33bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC915230.12348829617049333Illumina Paired End PCR Primer 2 (100% over 30bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT795100.10727963930941865Illumina Paired End PCR Primer 2 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)