FastQCFastQC Report
Thu 14 Sep 2023
EGAF00000286305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000286305
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences72411794
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT3124290.4314614826419022Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA3062530.4229324852799531Illumina Paired End PCR Primer 2 (100% over 45bp)
GTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC1090390.15058182372888043Illumina Paired End PCR Primer 2 (100% over 58bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC901750.12453081883318622Illumina Paired End PCR Primer 2 (100% over 55bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)