FastQCFastQC Report
Fri 15 Sep 2023
EGAF00000286306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000286306
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences77411708
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT7179510.9274449802864446Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA6530800.8436449948888869Illumina Paired End PCR Primer 2 (100% over 45bp)
GTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC2152350.27803933740875475Illumina Paired End PCR Primer 2 (100% over 58bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC1651300.2133139860445916Illumina Paired End PCR Primer 2 (100% over 55bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATG1218720.1574335499741202Illumina Paired End PCR Primer 2 (100% over 47bp)
AGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT1163150.1502550492749753Illumina Paired End PCR Primer 2 (100% over 54bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCT859330.11100775608774839Illumina Paired End PCR Primer 2 (100% over 59bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)