FastQCFastQC Report
Sun 14 May 2023
EGAF00000286310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000286310
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences55876230
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT3703070.6627272455568316Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA2731160.48878745040601346Illumina Paired End PCR Primer 2 (100% over 45bp)
GTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC1228140.21979650380850677Illumina Paired End PCR Primer 2 (100% over 58bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC905680.1620868122276682Illumina Paired End PCR Primer 2 (100% over 55bp)
AGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT629680.11269192642381207Illumina Paired End PCR Primer 2 (100% over 54bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATG600570.10748219770732564Illumina Paired End PCR Primer 2 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)