FastQCFastQC Report
Sat 13 May 2023
EGAF00000286312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000286312
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences50173657
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT2415310.4813900649099586Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA1412310.28148436539118527Illumina Paired End PCR Primer 2 (100% over 45bp)
GTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC735230.14653705628832278Illumina Paired End PCR Primer 2 (100% over 58bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC533360.10630279550880656Illumina Paired End PCR Primer 2 (100% over 55bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)