FastQCFastQC Report
Wed 15 May 2024
EGAF00000365003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000365003
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences46688329
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length95
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN869080.18614502138211028No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG821260.1759026329685091Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG718760.15394853818820545Illumina Paired End PCR Primer 2 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)