FastQCFastQC Report
Thu 16 May 2024
EGAF00000492975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000492975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51853342
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length75-100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC4970110.9584936685469568TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC694970.13402607685344564TruSeq Adapter, Index 4 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG561090.10820710456811058TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)