FastQCFastQC Report
Wed 15 May 2024
EGAF00000492976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000492976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51853342
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length75-100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3288540.6342002025636072Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC547830.105649892344451Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)