FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000494664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000494664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4499099
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT3540497.869331170529921TruSeq Adapter, Index 21 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84400.18759311586608785No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTAT71090.15800941477393585TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTTGGAATCTCGTAT57770.12840348700928786TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)