FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000494666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000494666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9989599
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3119823.1230683033423063TruSeq Adapter, Index 5 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2333792.3362199023204036No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACCTCGTATGC153100.15325940510725206TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC142230.14237808744875546TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGATCTCGTATGC116830.11695164140222244TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATACAGTGATCTCGTATGC100610.10071475341502698TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAATGATCTCGTATGC100410.10051454517844009TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)