FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000494667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000494667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022084
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC404130567.10808085705878TruSeq Adapter, Index 12 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82636413.722226392059625No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGTAATCTCGTATGC620631.0305900747980268TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGTAATCTCGTATGC311540.5173292169288903TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGTAATCTCGTATGC166790.2769639214597472TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTTATCTCGTATGC153140.2542973495554031TruSeq Adapter, Index 12 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC128640.21361375895786244TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTATAATCTCGTATGC126060.20932952778473365TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAAATCTCGTATGC121680.20205629811872436TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC116000.192624347318968TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGTTATCTCGTATGC115910.19247489739432397TruSeq Adapter, Index 12 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC104850.17410916221029132TruSeq Adapter, Index 6 (100% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG95120.15795196480155374TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTAATCTCGTATGC79560.13211373338531976TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC62160.10322008128747456TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGC61950.10287136479663851TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCTTGTAATCTCGTATG61730.10250604275861977Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC61160.1015595265692076TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)