FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000494671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000494671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4321760
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4022009.306393691459034TruSeq Adapter, Index 9 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN379190.8773971715227129No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC175480.4060382807004554TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCGTATGC72250.1671772611158417TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC59760.13827699825996817TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC56740.13128910443893227TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)