FastQCFastQC Report
Thu 5 Jan 2023
EGAF00000494672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000494672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12375434
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC280119022.635084959444654TruSeq Adapter, Index 6 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1999941.6160564550705858No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAAATCTCGTATGC315960.25531225813979536TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC186310.15054825551976603TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCAATATCTCGTATGC161510.13050855428585373TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGC139300.11256170894693471TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)