FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000494675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000494675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205134
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC111910526.612826131105454TruSeq Adapter, Index 9 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1705574.055923069276746No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC788341.8747083921701424TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGACACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC101930.24239417816412034TruSeq Adapter, Index 9 (100% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCGTATGC87740.20864971247051817TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC73940.17583268452325182TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGATCAGATCTCGTATGC73180.17402536994064874TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGATCAGATCTCGTATGC65450.15564307819917272TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGATCAGATCTCGTATGC64550.15350283724609015TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGATCAGATCTCGTATGC64390.1531223499655421TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGATCAGATCTCGTATGC61510.14627357891567783TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGATCAGATCTCGTATGC60240.14325346112632797TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC55120.13107786814879147TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGATCTCGTATGC52830.125632143945948TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATGC44600.10606082945275941TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)