FastQCFastQC Report
Sat 11 May 2024
EGAF00000502761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000502761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10997620
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC176245816.025812857691026TruSeq Adapter, Index 2 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5024044.568297504369127No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCATCTCGTATGC128050.11643428305396986TruSeq Adapter, Index 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)