FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000502764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000502764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6137889
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5554239.049088375498481TruSeq Adapter, Index 6 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122420.19944968050090187No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGCATGC63930.10415633127285294TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)