FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000502765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000502765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336640
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC30996013.2652013147083TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC236771.0132925910709394TruSeq Adapter, Index 12 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172220.7370412215831279No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCAATCTCGTATGC38410.16438133388112847TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTT35880.15355382087099426TruSeq Adapter, Index 12 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG31530.1349373459326212TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATTTCGTATGC26510.1134535058887976TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)