FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000502766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000502766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3750132
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT38396210.238626267022068TruSeq Adapter, Index 14 (97% over 44bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2293846.116691359130826No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT44520.11871582120309364TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGAATCTCGTAT40460.10788953562167944TruSeq Adapter, Index 14 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)