FastQCFastQC Report
Wed 8 May 2024
EGAF00000502767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000502767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5216794
Total Bases748 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT121768123.3415580527044TruSeq Adapter, Index 15 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2203314.223494353045185No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT221790.4251461721509417TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATTTCGTAT65410.1253835209900947TruSeq Adapter, Index 15 (97% over 40bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA64390.123428297149552TruSeq Adapter, Index 15 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)