FastQCFastQC Report
Wed 8 May 2024
EGAF00000502772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000502772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710787
Total Bases105.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAACCACCATGGCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG10130.14251808206959327Illumina Paired End PCR Primer 2 (100% over 33bp)
GCAAACCACCATGGCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGA7990.11241060964817871Illumina Paired End PCR Primer 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)