FastQCFastQC Report
Thu 9 May 2024
EGAF00000505089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000505089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7044474
Total Bases359.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC944781.3411647200344554TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG375260.5327012350389823TruSeq Adapter, Index 12 (100% over 49bp)
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA292030.4145518884731493No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG288240.4091717848628585No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT286330.40646043977165647No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT282350.4008106212046492No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT273640.38844631976780664No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG270240.3836198415949864No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC268850.3816466637537451No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG265840.3773738110183954No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG265780.37728863787416916No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA232210.3296342636795877No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG183940.26111246914957736No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA167200.23734916191045632No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT159890.22697223383889273No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC147410.20925621983983472No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT129640.18403077362482992No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC126740.17991407165389495No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC123050.17467592328398118No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC119850.17013335559191503No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGA118220.16781948517376882No Hit
TCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCAC116240.1650087714143029No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT113100.16055137686646298No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAA111710.15857819902522174No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT92540.13136537944493798No Hit
TCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCA88690.1259001026904209No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGA88370.12544584592121427No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTT81250.11533863280636708No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG80640.11447270584006698No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGG78600.1115768189363748No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA77990.1107108919700747No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC77270.1096888142393598No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA76160.10811311107117437No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGT74040.10510365997518055No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAA72810.10335761051854261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)