FastQCFastQC Report
Wed 1 May 2024
EGAF00000511725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25208774
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length75-76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGCT400680.1589446595062497No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT359870.14275585159357612No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC328490.13030780473497044TruSeq Adapter, Index 7 (100% over 50bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC328440.130287970370951No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC312480.12395684137594316No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG275450.10926751138313985No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG272040.10791480775701348No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG266080.10555055156589528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)