FastQCFastQC Report
Wed 1 May 2024
EGAF00000511727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29631129
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75-76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC788990.2662706507065593TruSeq Adapter, Index 7 (100% over 50bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT353310.1192360912066496No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC310930.10493356496811175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)