FastQCFastQC Report
Thu 2 May 2024
EGAF00000511728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29631129
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75-76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG430660.14534039523097483Illumina Single End PCR Primer 1 (100% over 50bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT316090.10667497684614041No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGG298370.10069477946655356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)