FastQCFastQC Report
Wed 1 May 2024
EGAF00000511730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29499545
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75-76
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGG507450.17201960233623942No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC366570.12426293354694115No Hit
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTT363080.12307986445214664No Hit
CGAGAACTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCC348960.11829334994827886No Hit
CAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGCT338270.11466956524244695No Hit
CCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAAC303270.10280497546657076No Hit
CAGGCATCCTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACC302110.10241174906257028No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG296710.10058121235429224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)