FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000511774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31844296
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGC540510.1697352643625722No Hit
CGAGAACTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCGACATCGTCTTCACCATCAA430980.1353397795322591No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAACAATGG393880.1236893414129802No Hit
GCCAGATCTTCCTGGGAGGTGGCTGAAGAGACTTAGGCTTAAGCCACGGGGGCCAGGCCGACCTGGTTGTTTGCC372480.11696914260563337No Hit
CAGGCATCCTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGAGA360680.11326361242214304No Hit
CTGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGATGGCTGAGCAGCTGACCACCATGTCGCCATC360120.11308775675241808No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGGGTGGCACGGGGTACTGGACTCCATT357660.11231524791755484No Hit
GTGAAATCCTGGGCAGGAGTTCCGATGCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGT355970.11178454062856344No Hit
CTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGAGACGGAACCT349020.10960204615608397No Hit
CAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATCACCACGCTGCCACTCTGGTCATCGGCGCTGAG348990.10959262531663441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)