FastQCFastQC Report
Wed 1 May 2024
EGAF00000511775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27343455
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGATGGCTG662680.24235415751228218No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC528460.1932674565083308No Hit
CTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTGGGTC508710.18604452144032274No Hit
TCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCT470540.1720850565519244No Hit
CGAGAACTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCC431790.15791347508937698No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG422200.15440623725129105No Hit
CTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGAT420750.1538759458159183No Hit
GTGAAATCCTGGGCAGGAGTTCCGATGCCGATAGTGCCGAAGTACTCCAT415910.1521058695764672No Hit
CTCAGAGCTGCTCTTCCTCTGTGGCCAGTTGGGGACCAGCATCATGAAGT389510.14245090827037038No Hit
CAGGCATCCTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACC368150.13463916684998292No Hit
CTTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGCACC356140.13024689089217145No Hit
CAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTGGAGGAGCCGGTG353150.12915339338060972No Hit
GCCAGATCTTCCTGGGAGGTGGCTGAAGAGACTTAGGCTTAAGCCACGGG345030.12618376134252235No Hit
CAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATCACCACGC331090.1210856491983182No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG325360.11899008373301764No Hit
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTT322920.11809773124866628No Hit
CTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTGGGGC311070.11376397020786144No Hit
CTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTCTTCTCTG307100.11231206882963402No Hit
CCAGGTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGCC287710.1052207923248909No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG286830.10489896028135434TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)