FastQCFastQC Report
Wed 1 May 2024
EGAF00000511776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27343455
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGATGGCTG540190.19755733136138065No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC519060.18982970513419026No Hit
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTT420560.1538064593519729No Hit
CGAGAACTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCC391250.14308725799281766No Hit
CTTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGCACC385460.1409697494336396No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG376980.1378684588322873No Hit
CTCAGAGCTGCTCTTCCTCTGTGGCCAGTTGGGGACCAGCATCATGAAGT360200.13173170691121514No Hit
GTGAAATCCTGGGCAGGAGTTCCGATGCCGATAGTGCCGAAGTACTCCAT356980.13055409420645636No Hit
CTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGAT356750.1304699790132593No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC333120.12182805720784004Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTGGAGGAGCCGGTG308890.11296670446364589No Hit
CAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATCACCACGC306440.11207069479698159No Hit
GCCAGATCTTCCTGGGAGGTGGCTGAAGAGACTTAGGCTTAAGCCACGGG304030.11118931385956894No Hit
CAGGCATCCTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACC299950.10969718347589943No Hit
CTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTCTTCTCTG291520.10661417878611171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)