FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000511776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000511776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27343455
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAACAATGG401090.1466859253887265No Hit
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGC391090.143028743075811No Hit
CGAGAACTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCGACATCGTCTTCACCATCAA337010.12325070112756417No Hit
GTGAAATCCTGGGCAGGAGTTCCGATGCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGT326750.11949843207451288No Hit
CTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGAGACGGAACCT305660.11178543457657417No Hit
CTGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGATGGCTGAGCAGCTGACCACCATGTCGCCATC283100.10353483127863689No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGGGTGGCACGGGGTACTGGACTCCATT281100.1028033948160538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)