FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000525387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000525387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62967515
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC2185680.3471123165651368No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG1832080.29095637647444084No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA1772390.28147688534318055No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG1737850.2759915172132805No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA1645660.2613506345295666No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA1556380.2471718949048569No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC1421780.22579579327531027No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1345760.21372290140400174No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA1337520.212414290130395No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1334230.21189179849323891No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC1239050.19677606778669923No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1122070.17819823443882135No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA1109920.17626866805844252No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA1072120.17026557265281947No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG1057490.16794215239397647No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA1039970.16515976531708454No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC1012750.16083690137684487No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG954660.15161150952201305No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG929050.14754433297867955No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA908640.14430297908373865No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTC906290.1439297707714843No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC859070.13643066587588853No Hit
CCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGT855830.13591611484112084No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT845390.13425811706242496No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC844680.1341453605085098No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC832610.13222849909195242No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA797300.1266208456852712No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG795030.12626034233683828No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA789590.12539640479698144No Hit
CTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCC789520.12538528795363768No Hit
CTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCA777010.12339854923606244No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG773410.12282682586409835No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA748100.11880729293509518No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG740040.11752726783008666No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA737880.1171842338069082No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA726300.11534519029375702No Hit
CCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG719870.11432402882661005No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT710870.1128947203966998No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC698500.11093021536581205No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT695060.11038390192149078No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT693000.1100567491030891No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG689650.10952472874306697No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC676990.10751416821832656No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT666010.10577041193383604No Hit
AATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGA659770.10477942475576495No Hit
CGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA642300.10200497828126139No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC641260.10183981375158285No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTC639940.10163018184852934No Hit
CATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTA635870.10098381681411439No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC633120.10054708368275292No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC631950.10036127358686459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)