FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000525650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000525650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22328213
Sequences flagged as poor quality0
Sequence length42
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC7559543.385644878969938TruSeq Adapter, Index 5 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACANTGATC2625261.1757591169521717TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGNGATC1227650.5498200863633825TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTNATC1019580.4566330498549077TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACANNGATC546090.24457398359644814TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACANNNATC417670.18705930474597318TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)