FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000525651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000525651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44860461
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC8831281.9686110671042816TruSeq Adapter, Index 9 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATNAGATC1794360.3999869729381515TruSeq Adapter, Index 9 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCANATC862430.1922472441823547TruSeq Adapter, Index 9 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGNTNANATC488230.10883303227757736TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)