FastQCFastQC Report
Sat 25 May 2024
EGAF00000583953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000583953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79085558
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC3673170.46445521696894393TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC3296970.41688648134720124TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)