FastQCFastQC Report
Wed 6 Sep 2023
EGAF00000586344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000586344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61256385
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11308101.84602796916599Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT3476630.5675538966264496Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG1730520.28250442790576036Illumina Paired End Adapter 2 (96% over 28bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG752920.12291290124286636Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)