FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000588544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000588544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44148
Sequences flagged as poor quality0
Sequence length101
%GC19

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANANNNNNAAANANAAAN7021.5901060070671376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANAAANNAANANNNNNAN5471.2390142248799494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANNANAA5331.2073027090694937No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5091.1529401105372836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANAAAANNNNNNNAANAA4380.9921174232128296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAANNAAANAANNNNNNANNNNNNN4210.9536105825858476No Hit
AAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.9377548246806198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANAAAAAAANAANNNNA3350.7588112711787623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANANANAANN3170.718039322279605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAN2390.541360877049923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAANAAANANAAAAAANNANNNNNAN2340.5300353356890459No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAANNNNAANNNANNAN2250.5096493612394672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNAAAAAANANNNAAANNNA2210.5005889281507656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNAA1940.43943100480202957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANACAANNNNNNNAANAA1630.36921264836459183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANNNNNN1430.32391048292108365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAANANNNNNNANNNN1420.3216453746489082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANAACANNNNNNNAANAA1270.28766875056627705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAANAAAANN1060.24010147685059346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAAAANAANANNNANNNA1050.23783636857841803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANANA1050.23783636857841803No Hit
AAAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.23104104376189183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAANNNAANNNN920.2083899610401377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAAANAAAAA910.2061248527679623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNAAAANANNANANAN840.19026909486273444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAAANNNCNNNNCNNN830.18800398659055903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAANAAANNNNNANNNNNNNNNNNNN820.18573887831838362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAANAANNANAA820.18573887831838362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAAA820.18573887831838362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAANAAANAAAANN810.1834737700462082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAANAAAANANANANNNNNNNNNNNNN790.1789435535018574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANANNNNNAAANANACAN780.17667844522968199No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANNANAC780.17667844522968199No Hit
AAAAAAAAAAAAAAAANAAAAAAAAAAAAANAAANNCANNNNNAANANNN750.16988312041315573No Hit
AAAAANAAAAAAAAAAAAAAAAAAAANNNANNNANAANNAAANNNNNAAN670.15176225423575246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANACAAAAANAANNNNA660.14949714596357705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANN660.14949714596357705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNANANNNNNNNNNNNNN650.14723203769140164No Hit
AAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTTTTNTTTTT610.1381716046027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAANAANNANAA610.1381716046027No Hit
AAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN600.1359064963305246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANNNANAANN580.13137627978617378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAANAANANANNNNNNNNNNNNNNN540.12231584669747216No Hit
AAAAAAAAAANAAAAAAAAAAAAAAANAAAANAAAAAAANAAAAANNNAN520.11778563015312131No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANANAANANNNANNNANN480.10872519706441967No Hit
AAAAAAAAAAAAAATTTTTTTTTTTTTANNTTTNTTNNNNNNTNNNNNNN450.10192987224789343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAANACAANNNNNNNCANAA450.10192987224789343No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAANNAAANAANNNNNNANNNNNNN450.10192987224789343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)