FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000590575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590575
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences88309147
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT13404671.5179254307597378No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT11293171.2788222266488432No Hit
AGCTTTTTTGAAAGTTAATCTCCTGGTTCACCGTCTGCTCGTACGCGGTT6700520.7587571874066454No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAGCGCCGGCCGGCG6624850.7501884261207958No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT4386570.49672883829350084No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG4223520.47826529226921416No Hit
AGCTTTAACAACCACGTGCGCACGCTTGTGGCGCGCGAGGATATAATTAA4091900.46336083395755145No Hit
AGCTTTTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAA3989850.45180483965041585No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGG3914030.4432190925816552No Hit
AGCTTAAGGTGTCGCCGCGAATGGCATGATCGTTCATGTCCTCCACCGGC3781110.42816742415143016No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG3712670.42041737760189213No Hit
AGCTTAAAAGATGACACCGAACAGGGCGGGGATGGCGCAGGCGGCGGCAA3599110.407558007552717No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG3443440.3899301620476529No Hit
AGCTTGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCCTCGCG3237220.3665781077015725No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT3159010.3577217204917629No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA3067750.34738757016869387No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC2825500.3199555307673847No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT2665150.3017977288354965No Hit
AGCTTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCT2659440.30115113669935006No Hit
AGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTG2645760.2996020332978644No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2448360.2772487429869524No Hit
AGCTTGCATGCCAGAGGCCAGGCCATCTACCACCTTTTGTTGGAAATTTG2415670.27354697469787587No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGGGGCCGGCGGG2312320.26184377027217803No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCTTCCACCGAATTTTAATATG2297190.2601304709692191No Hit
AGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTG2200210.2491485961244762No Hit
GATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC2125040.2406364541149967No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT1905200.21574209068059508No Hit
AGCTTGTAGAGTTACTTGAATTGCTCATATACTTAATTAAAAAATCCCAG1872800.21207316157181316No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCG1855770.21014470901864787No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC1835380.20783577492827554No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG1637020.18537377560673302No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT1574600.17830542514469086No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1557520.17637131066388853Illumina Single End PCR Primer 1 (100% over 50bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1474560.16697704032856303No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT1407900.1594285584029025No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1316670.14909780523641566No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG1291800.14628156242976734No Hit
AGCTTGATCCCCGCCCTCCCGTAGAGGAGCCTCCACCGGCCGTGGAGACA1276890.1445931756084112No Hit
CGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAGGA1274340.14430441729892374No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1226890.1389312479714021No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGGCGAGGCAGCGGCAGAAGG1220220.13817594682462508No Hit
AGCTTATTTAAATAATATAAGCGCGCTGAGGCCGAGGCAGCGGCAGAAGG1208360.13683293758912654No Hit
CCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTC1170680.13256610892187645No Hit
CCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCG1127830.12771383693695965No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG1122930.12715896802853277No Hit
AGGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATAC1122120.12706724480081322No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG1111050.1258136940219794No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT1098170.12435518146268584No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1053690.11931833063680256No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC1025950.11617709318378991No Hit
GGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGG1005290.11383758468417773No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC921310.10432781102505723No Hit
AGCTTGCCTGGTAGGCGTCCACATCCAACAGTGCGGGAATGTTACCACCC919530.1041262464011797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)