FastQCFastQC Report
Thu 23 May 2024
EGAF00000590591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590591
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences100822256
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC34060073.3782293068308253Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC3751650.3721053415031697No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG3418930.33910469132926363No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT3373640.33461262759286003No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC3080970.3055843146378316No Hit
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCT1828060.1813151255016551No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1504230.14919622508744498No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC1302970.1292343626986486No Hit
GATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC1300710.12901020584185302No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC1212030.12021452882387397No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGT1122470.11133156948997451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)