FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000590599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000590599
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences44771546
Sequences flagged as poor quality0
Sequence length90
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT2012180.44943277143031873No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGT1677430.37466430129529144No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT1675840.3743091650219092No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGA1516300.3386749253644268No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1449180.3236832607924685Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1316120.29396349190175386No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT1308220.2921989783421819No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT1256390.2806224292545091No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC1130630.25253316023529765No Hit
CAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT1082180.24171155492374555No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA1076840.2405188330999336No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1076400.24042055639534984No Hit
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCT1041650.232658930294701No Hit
AGCTTTTTTGAAAGTTAATCTCCTGGTTCACCGTCTGCTCGTACGCGGTT996500.22257440026752706No Hit
AGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAGCGCCGGCCGGCG987280.22051505659420384No Hit
CGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGG968130.21623778638334268No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA950390.2122754483394431No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT918210.2050878475360221No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC885260.19772826249957956No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT866440.1935246998171562No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA862320.19260447249241738No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA854100.19076848496587542No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA852010.19030167061910258No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG843440.18838750844118718No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA842660.1882132906466978No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA768880.1717340741371763No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG724980.16192873929347895No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC723910.16168974821642298No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG719410.1606846455559073No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTA705080.15748395197253184No Hit
AGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAG697420.15577304388818738No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTC678060.15144886888650214No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG648230.14478615502801712No Hit
AGCTTAAGGTGTCGCCGCGAATGGCATGATCGTTCATGTCCTCCACCGGC643460.1437207462078705No Hit
AGCTTAAAAGATGACACCGAACAGGGCGGGGATGGCGCAGGCGGCGGCAA627010.14004653759331875No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA614040.1371496083695658No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT612630.13683467620260423No Hit
AGCTTTAACAACCACGTGCGCACGCTTGTGGCGCGCGAGGATATAATTAA612190.13673639949802047No Hit
CGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGC607770.13574916532924727No Hit
AGCTTTTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAA605030.13513716948706664No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA603970.1349004119714785No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC598820.13375012781555498No Hit
CAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAA597510.13345753126327153No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCT592040.13223577314037804No Hit
AGCTTATCGATGATAAGCTGTCAAACATGAGAATTCTTGAAGACGAAAGG589970.13177342591654084No Hit
GGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCC587260.13116813075876363No Hit
GGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTG571980.12775524883594594No Hit
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA569370.12717228929284685No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG564610.12610911403416802No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC563490.12585895514977302No Hit
CCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGC558180.12467293401036454No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG552440.1233908697278401No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT543540.12140300002148685No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGG542900.12126005208754685No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG538280.12022814668941743No Hit
CGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGG536910.1199221487683271No Hit
TGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATG536870.11991321452245585No Hit
AGCTTGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCCTCGCG509990.11390940129697555No Hit
CGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACGCGC496340.11086058989341133No Hit
AGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGA489770.10939314000905843No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG488360.10907820784209685No Hit
CATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCT484560.10822945448432807No Hit
CGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGT481750.10760182371187271No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT478390.10685134705868768No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG476770.10648951010090203No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG465690.10401472399456566No Hit
CTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT464220.10368639045879721No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC461310.10303642407166373No Hit
CGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTT450220.10055940440385953No Hit
CCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAAC449210.10033381469561048No Hit
CGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGA448930.10027127497451171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)